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Fig. 5 | BMC Cancer

Fig. 5

From: Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells

Fig. 5

KAT5 silencing induces MET and TSPAN13 mRNA expression in MDA-MB-231 in vitro. a Quantitative and qualitative analysis after transfection with KAT5 SMARTpool of E-CAD induction and cell morphology. b Quantitative and qualitative analysis after transfection with four individual siRNA and the KAT5 SMARTpool in MDA-MB-231 cells. Data are presented as the mean ± sd of 2 independent experiments each with 3 biological replicates (* p < 0.05, **p < 0.01, ***p < 0.001 compared with siScr determined by Wilcoxon-Mann Whitney’s test). c RT-qPCR quantification of KAT5, E-CAD and TSPAN13 transcripts using specific primers in MDA-MB231 cells transfected with individual siRNA 1 (10nM) and 2 (1nM). The columns represent the mean expression ± sd of 2 independent experiments normalized to siScr. d Individual siRNA 1 (10nM) reduced the migration compared with siScr or miR-200c transfected cells. Data are presented as the mean ± sd of 2 independent experiments each with 2 biological replicates. Scale bars, 100 μm

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