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Fig. 2 | BMC Cancer

Fig. 2

From: Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells

Fig. 2

Cell based assay development and validation. a MDA-MB231 cells were transfected with a pre-miRNA negative control (ctrl; 5nM) or pre-miR-200c (5nM). Phase contrast images were taken at 6 days after 2 successive transfections (magnification, ×10). b MDA-MB-231 cells were transfected as in A with a pre-miR negative control scramble (ctrl), pre-miR-200a (200a), pre-miR-200c, mix of pre-miR200a + pre-miR-200c (200a/c) (10 nM) and after 6 days the expression of E-CAD and GAPDH were studied by RT-qPCR. Non treated cells (NT); Mock-transfected cells (Mock). c Immunofluorescence staining of E-CAD (green) and texas-red phalloidin staining of F-ACTIN (red) in cells transfected as in (a) with scramble (siScr), pre-miR-200c and a ZEB1-specific siRNA pool (10 nM). Cells are counterstained with DAPI (blue) to visualize nuclei. Scale bars, 100 μm

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