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Fig. 1 | BMC Cancer

Fig. 1

From: Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells

Fig. 1

Screening strategy. A four step process was used to identify MET inducer gene candidates. a The primary screening was performed on 729 siRNA pools targeting 729 genes selected for chromatin structure maintenance. From E-CAD induction measurements and cell morphology observations, 70 pools were identified. b Deconvolution analysis: E-CAD induction and cell morphology were analysed for each siRNA contained in the active pools. c Transcript quantification was done by RT-qPCR to control gene knockdown. d Cell migration study: the lost of the mesenchyme phenotype was associated to impaired migration capabilities for several siRNA

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