Fig. 2

4T1-derived tumors have increased necrotic area, proliferation and blood vessels in Lgals3−/− mice. Quantification of a the necrotic area and b Ki67 in 4T1 tumors inoculated in Balb/c Lgals3+/+ or Lgals3−/− mice 21 and 28 days p.o.i. c Representative immunohistochemical staining of CD31 and (d) quantification of the vascular lumen area in 4T1 tumor inoculated in Balb/c Lgals3+/+ or Lgals3−/− mice 21 and 28 days p.o.i. Data are the mean ± S.D., n = 4, three animals per group; *p < 0.05, **p < 0.01, ***p < 0.001