Performance and prognostic utility of the 92-gene assay in the molecular subclassification of ampullary adenocarcinoma

Table 1 Clinicopathologic features of ampullary adenocarcinomas

Clinicopathologic feature	No.	Percent	Median OS (months)	Log rank P
Sex				0.23
Male	35	64.8	103.8
Female	19	35.2	245.2
Age, years				0.16
Mean	64.6
Median	66.5
Range	34-83
T stage^a				0.15
T1	6	11.3	NA
T2	17	32.1	103.8
T3	21	39.6	39.7
T4	9	17.0	108.1
N stage				0.034
N0	24	44.4	113.8
N1	30	55.6	41.8
Grade				0.92
I	2	3.7	113.8
II	28	51.9	63.7
III	24	44.4	108.1
Mucinous				0.17
Negative	50	92.6	108.1
Positive + signet ring	4	7.4	45.6
Perineural invasion				0.70
Negative	44	81.5	108.1
Positive	10	18.5	94.8
Lymphovascular invasion				0.46
Negative	38	70.4	113.8
Positive	16	29.6	57.2
Perioperative treatment				0.94
Neoadjuvant +/or adjuvant	25	46.3	145.9
None	29	53.7	103.8
Adenoma precursor lesion				0.56
Absent	33	61.1	113.8
Present	21	38.9	63.7
Resection margin				0.006
R0	52	96.3	108.1
R1	2	3.7	18.8
CK7 expression				0.70
Negative	12	22.2	63.7
Positive	42	77.8	108.1
CK20 expression				0.97
Negative	34	63.0	113.8
Positive	20	37.0	103.8
CDX2 expression^b				0.93
Negative	33	61.1	108.1
Positive	21	38.9	103.8
MUC1 expression^c				0.28
Negative	19	35.2	113.8
Positive	35	64.8	63.72
Histopathologic subtype^d				0.035
Intestinal (plus 8 mixed histology)	28	52.0	108.1
Pancreaticobiliary (plus 6 mixed histology)	26	48.0	34.6
Histomolecular phenotype				0.017
Non-Pancreaticobiliary	40	74.1	113.8
Pancreaticobiliary	14	25.9	28.0
92-gene Classifier^e				0.050
Intestinal	21	38.9	108.1
Pancreaticobiliary	31	57.4	36.4

Abbreviations: OS, overall survival
^aT stage, N = 53, one case was carcinoma in situ
^bPositive CDX2 expression was defined as modified H score > 35
^cPositive MUC1 expression was defined as any positive staining
^d P value for the predominant subtype. The predominant subtypes for the 14 samples with mixed histology are also indicated
^eSingle gastroesophageal and single lung adenocarcinoma predictions excluded. P < 0.05 was considered statistically significant

ISSN: 1471-2407