Fig. 8
Effect of GIRK1 variant overexpression on single cell motility coefficients. a Left panel: Flower plots showing the trajectories of 5 representative cells for the entire observation interval of 72 h (black line). Starting position of each individual cell was set to zero coordinates (black circle). Grey circles indicate the positon of an individual cell after 72 h. Right panel: Squared distance as a function of time for the five cells shown to the left (circles; bars indicate standard error). Solid lines represent a linear fit through the data, resulting in the motility coefficient (MC). Upper: MCF-7eYFP; lower: MCF-7GIRK1a. b Graphical representation of cellular motility coefficients for different experimental groups. WT: MCF-7WT; eYFP: MCF-7eYFP; hG1a: MCF-7GIRK1a; hG1c: MCF-7GIRK1c; hG1d: MCF-7GIRK1d. The median value is represented by the black line within the box, box margins represent 75 and 25 % percentiles, whiskers indicate 90 and 10 % percentiles, values outside the 10–90 % interval are plotted individually as crosses. The grey line represents the mean value. The number of individual cells is given in parenthesis besides each box. Statistical significant differences between groups are indicated. hG1a, as well as hG1c, differs from both WT, as well as from eYFP, statistically significant at the p < 0.001 level. hG1d differs from both hG1a, as well as from hG1c, statistically significant at the p < 0.001 level. Kruskal-Wallis one way analysis on ranks was used for analysis of statistical significance