Fig. 6

Effects of GIRK1 variant overexpression on chemoinvasion through Matrigel. a Comprehensive view of fixated cells, stained with crystal violet, that managed to invade the Matrigel layer (10x magnification). The Scalebar corresponds to 100 μm for both images. Upper: MCF-7^GIRK1a, lower: MCF-7^GIRK1d. b Quantification of the number of invasive cells after 24 h. WT: MCF-7^WT; eYFP: MCF-7^eYFP; hG1a: MCF-7^GIRK1a; hG1c: MCF-7^GIRK1c; hG1d: MCF-7^GIRK1d. Mean values ± SEM were plotted (number of experiments is given in parenthesis above each bar). Statistical significant differences between groups are indicated. hG1d differs from eYFP statistically significant at the p < 0.05 level. hG1a and hG1c differ from hG1d statistically significant at the p < 0.001 level. Kruskal-Wallis one way analysis on ranks was used for analysis of statistical significance