Fig. 2
Overexpression of GIRK1 mRNA and protein in the stably transfected MCF-7 cells assessed by IHC. a Quantification of mRNA expression encoding different GIRK1 variants via qPCR in stably transfected cells. Expression was normalized to the expression level in the MCF-7WT cell line. WT: MCF-7WT; eYFP: data derived from two cell lines with stably integrated pEYFPN1 vector alone (MCF-7eYFP); hG1a: data derived from two cell lines overexpressing N-terminal and two cell lines overexpressing C-terminal fusions of eYFP with the GIRK1a protein (MCF-7GIRK1a); hG1c: data derived from two cell lines overexpressing C-terminal fusions of eYFP with the GIRK1c protein (MCF-7GIRK1c); hG1d: data derived from two cell lines overexpressing N-terminal fusions of eYFP with the GIRK1d protein (MCF-7GIRK1d). Mean values ± SEM were plotted (number of experiments is given in parenthesis above each bar). Statistical significances between groups are indicated. hG1a, as well as hG1d differs from both WT, as well as from eYFP, statistically significant at the p < 0.001 level. hG1c differs from both WT, as well as from eYFP, statistically significant at the p < 0.05 level. Kruskal-Wallis one way analysis on ranks was used for analysis of statistical significance. b Detection of GIRK1a protein in stably transfected cells via immunohistochemistry. Brown: Immunoreactivity. Blue: hematoxylin stain. Scale bar corresponds to 100 μm throughout. Upper image: MCF-7WT; Lower image: MCF-7hGIRK1a