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Fig. 1 | BMC Cancer

Fig. 1

From: Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner

Fig. 1

cLSM in-vivo reveals overexpressed GIRK1 protein to localize in a large part to the ER. Horizontal sequences of images show identical cells. The sequence of channels (from left to right is: eYFP (green)/ eCFP (red)/ overlay / transmission. Scalebar : 15 μm in all images. a Upper sequence: subcellular localization of GIRK1a, labelled with eYFP at the C-terminus (MCF-7GIRK1a; transient transfection with GpI-eCFP was used as marker for lipid rafts in the plasma membrane). Lower sequence: subcellular localization of GIRK1a, labelled with eYFP at the C-terminus (MCF-7GIRK1a; transient transfection with Srß-eCFP was used as ER marker). b Upper sequence: subcellular localization of hGIRK1c, labelled with eYFP at the C-terminus (MCF-7GIRK1c; transient transfection with Srß-eCFP was used as ER marker). Middle sequence: subcellular localization of hGIRK1d, labelled with eYFP at the N-terminus (MCF-7GIRK1d; transient transfection with Srß-eCFP was used as ER marker). Lower sequence: subcellular localization of eYFP alone (MCF-7eYFP; transient transfection with Srß-eCFP was used as ER marker)

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