Fig. 1

Measurement of DNA damage and cell proliferation in GSCs following IR. a 10 GSC lines were irradiated at 4Gy and subjected to comet assay at the indicated time. Data are given as a percentage of olive tail moment (OTM) and normalized to control (***p < 0.001 versus control cells). b GSCs from group 2 were irradiated at the indicated doses and subjected to comet assay immediately thereafter. Data are given as a percentage of olive tail moment (OTM) and normalized to control (***p < 0.001 versus control cells). c GSCs from group 2 were irradiated at 12Gy and subjected to comet assay at the indicated time. Data are given as a percentage of olive tail moment (OTM) and normalized to control (***p < 0.001 versus control cells). d Cell proliferation was measured 7 days after IR (4Gy and 12Gy) using an MTS assay (T0 = IR). Each set of results was obtained from three independent experiments. Experiments were performed in sextuplicate and expressed as mean ± SD. Doubling times were extrapolated based on exponential growth equations