Fig. 1

Expression of CK1 - isoforms during melanoma progression. a Relative mRNA expression (SYBR green real-time PCR) of three CK1 isoforms in melanocytic cells, namely normal human melanocytes (NHM), cell lines derived from primary radial growth phase (RGP) plus vertical growth phase melanoma (VGP) and cell lines from metastatic melanoma (MM). Normalized data (to ACTINB) are presented as scatter plot (mean with SEM). Kuskal-Wallis statistics with Dunn’s multiple comparison was used to test for significant differences (* p < 0.05; ** p < 0.01). b CK1α, δ and ε protein expression was determined by western blot analyses. Semi-quantification (ratios CK1/β-actin) are shown as scatter plots. Kuskal-Wallis statistics with Dunn’s multiple comparison was used to test for significant differences (* p < 0.05; ** p < 0.01). c Relative mRNA expression of three CK1- isoforms of patient-derived tissue samples. The analysis of CK-1 isoform expression was performed using benign melanocytic nevi (n = 4), primary malignant melanomas (n = 9), and metastatic melanoma (n = 13) by quantitative real-time PCR. Normalized data are presented as scatter plot (mean with SEM) and Kuskal-Wallis statistics with Dunn’s multiple comparison was used to test for significant differences (* p < 0.05; ** p < 0.01). d CK1α (blue), δ (green) and ε (red) expression in tissue sections of benign nevi (n = 11), primary melanomas (n = 11) or melanoma metastases (n = 16) was determined by immunofluorescence staining followed by confocal analysis. Kuskal-Wallis statistics with Dunn’s multiple comparison was used to test for significant differences (* p < 0.05; ** p < 0.01)