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Fig. 3 | BMC Cancer

Fig. 3

From: Recapitulating epithelial tumor microenvironment in vitro using three dimensional tri-culture of human epithelial, endothelial, and mesenchymal cells

Fig. 3

Immunohistochemical staining of STEM cryo-sections at day 15 (left panel): (a) fibronectin, (b) CK-18, (c) vimentin, and (d) CD-31, and (right panel) 2-D tri-culture controls. A549 population indicated by red arrowhead, HPMEC population indicated by green arrowhead, and MSC population indicated by blue arrowhead. Scale bar for low magnification images – 100 μm, scale bar for inset – 50 μm. Negative controls are shown in Additional file 1: Figure S1

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