Fig. 1

Silencing PRMT5 and p44 expression suppresses cellular proliferation and arrests cell cycle at the G1 phase. a, b Silencing p44 or PRMT5 expression in A549 cells. DNA microarray analysis of PRMT5 and p44 mRNA (a) or Western blot of p44 and PRMT5 protein levels (b) in A549 cells expressing NT, p44, or PRMT5 shRNA. Relative mRNA or protein levels = signals in cells expressing target shRNA/signals in cells expressing NT shRNA. c Silencing p44 or PRMT5 inhibited cell growth. Growth curves of parent A549 cells or A549 cells expressing NT shRNA, p44 shRNA, or PRMT5 shRNA. d Silencing p44 or PRMT5 suppressed cellular proliferation. A549 cells expressing NT shRNA, p44 shRNA, or PRMT5 shRNA were allowed to grow in the presence of BrdU and immunostained with the anti-BrdU antibody (brown). The black arrows (right two panels) indicate the BrdU-positive stained cells. d Percentage of BrdU-positive cells in A549 cells infected with NT, PRMT5, or p44 shRNA-expressing lentivirus. e Percentage of BrdU-positive A549 cells that expess NT, p44, or PRMT5 shRNA. f Silencing p44 or PRMT5 arrested cell cycle at the G1 phase. The cell-cycle distribution in A549 cells expressing NT shRNA, p44 shRNA, or PRMT5 shRNA by using flow cytometric analysis