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Fig. 1 | BMC Cancer

Fig. 1

From: S6Ks isoforms contribute to viability, migration, docetaxel resistance and tumor formation of prostate cancer cells

Fig. 1

S6Ks increase cell viability in PC3-luc cells. Western blotting analyses of S6K isoforms expression modulation in human prostate cancer cell line PC3-luc transduced with a lentiviruses pLKO.1-shRNAs against S6K1 and S6K2 and b with retroviruses carrying pBABE construction. S6K1 antibody detects both p85-S6K1 and p70-S6K1 proteins. c Relative cell viability in PC3-luc cells with knockdown of S6Ks isoforms and d in PC3-luc cells overexpressing S6Ks isoforms. Cells were seeded in 96-well plates at a density of 104 cells/well. After 24, 48 and 72 h, cells were treated with MTT (12 mM) for 4 h and absorbance was measured at 570 nm. *p < 0.05, **p < 0.01, ***p < 0,001, n = 3

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