Fig. 3

Characterization of AKT1-mutant and wild-type samples. a Mutant allele frequencies for AKT1 ^E17K mutations as identified by next-generation sequencing and BEAMing (Pearson correlation coefficient = 0.9). b Comparison of mutant versus wild-type AKT1 samples shows significantly different mutation spectra. Shown are mutations in genes that are distributed significantly differently between groups (P < 0.05, Fisher’s test, not corrected for multiple testing). Four of the seven PIK3CA mutations in mutant samples have low mutant allele frequencies (≤3 %). c AKT1-mutant samples as characterized by next-generation sequencing. All additionally mutated genes are shown that have known functional impact (gray box, known single nucleotide variants or indels) or likely functional impact (blue box, likely single nucleotide variants or copy number alterations or rearrangements). Yellow boxes indicate variants with unknown somatic/functional status in the listed genes with alterations of known or likely impact. Variants with unknown somatic/functional status found in additional genes of the FoundationOne® T5a panel are not depicted. Abbreviations: AKT1 v-akt murine thymoma viral oncogene, BEAMing Beads, Emulsions, Amplification, and Magnetics