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Fig. 5 | BMC Cancer

Fig. 5

From: Chemotherapy and radiation therapy elicits tumor specific T cell responses in a breast cancer patient

Fig. 5

Increased correlation of TCR Vβ families between tumor infiltrating lymphocytes and HER2/neu tetramer CD8+ T cells after chemotherapy. a. Dot plot example of an ex vivo flow cytometry analysis of TCR-Vβ families 13.2, 4 and 7.2 in CD8+ T cells HER2/neu369–377 tetramer negative vs. tetramer positive from before and after anti-TTx cells, each gate represents one TCR-Vβ family, PE positive correspond to Vβ13.2, the double positive cells corresponds to family Vβ4, and FITC positive corresponds to family Vβ7.2, numbers correspond to percentage of each family, left; Delta of the percentage of each of 24 families (after minus before therapy) in total CD8+ T cells (empty bars) or tetramer positive CD8+ T cells (black bars), right. b. Heat map of sequenced TCRs from total PBMCs (before and after anti-TTx) and Tumor Infiltrating Lymphocytes (TILs) from FFPE tumor tissue slice. Arrows points insert of highly expressed of TCR Vβ families and genes in TILs. c. Correlation plots of Vβ families of HER2/neu369–377 tetramer positive cells (red corresponds to before anti-TTx sample, and blue corresponds to after anti-TTx sample) analyzed by flow cytometry vs. the percentage of Vβ families obtained from TILs. Pre-chemotherapy r2: 0.164, p value: 0.017, post-chemotherapy r2: 0.477, p value: <0.0001 (linear regression analysis)

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