Fig. 3
Anti-TTx induces enrichment of CTLA-4 in tumor specific T cells. a. Expression of tetramer positive (pool of Her2/neu or viral tetramers) in CD8+ T cells after 6 days of stimulation with aDCs derived in situ and pulsed with a combination of HLA-A*02:01 restricted peptides (TAA peptide pool HER2/neu369–377, HER2/neu689–697 and HER2/neu435–443, or viral peptide pool CMVPP65, EBV280–288, and FLU58–66). b. Pie chart of naïve and memory sub-populations distribution in tetramer positive CD8+ T cells (TAA or viral specific) after stimulation with pulsed 2d-aDCs before and after anti-TTx (Blue; naïve, Grey; TCM, yellow; TEM; and green TEMRA). c. Fold increase of inhibitory receptors (PD1, BTLA, and CTLA-4) expression between tetramer positive over tetramer negative CD8+ T cells after stimulation with peptide pulsed 2d-aDCs, obtained before (white bars) and after (black bars) anti-TTx