Gene | PBS + GCV | BF/GCV | Ratio |
|---|
Bad | 335.83 | 982.79 | 2.93 |
Bax | 516.54 | 1567.29a | 3.03 |
Bim | 192.61 | 1488.21 | 7.73 |
Bcl-2
|
192.61
|
968.65
a
|
5.03
|
Bcl-w
|
192.61
|
1342.08
|
6.97
|
IGF-1
|
192.61
|
984.35
|
5.11
|
IGF-2
|
192.61
|
997.97
|
5.18
|
Caspase 3 | 192.61 | 1262.99 | 6.56 |
Caspase 8 | 192.61 | 1708.19 | 8.87 |
HtrA2 | 192.61 | 1314.83 | 6.83 |
Fas | 538.63 | 1201.19 | 2.23 |
FasL | 521.06 | 1154.57 | 2.22 |
P53 | 489.92 | 1175.53 | 2.40 |
P27 | 620.96 | 1405.44 | 2.26 |
- The boldface letters were anti-apoptosis proteins and the others were apoptosis proteins
- aThe ratio of Bax/Bcl-2 is 0.60
- Up-regulation of fourteen differential proteins expression was more than doubled in the BF + GCV group unlike the expression in the PBS + GCV group. To summarize, the anti-apoptosis proteins were increased more than 5.0-fold. The total ratio of pro-apoptosis to anti-apoptosis proteins was 2.02-fold and the ratio of Bax/Bcl-2 was 0.60. The typical mitochondrial control signal molecule, Cytochrome C (Cyto C), was not significantly changed in group BF + GCV. The results suggested that bifidobacterium itself (not GCV) induced cancer cell apoptosis via Fas/FasL signaling pathway without mitochondrial alteration and up-regulated P53 expression
