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Table 2 Apoptosis associated proteins change in group BF + GCV

From: Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors

Gene

PBS + GCV

BF/GCV

Ratio

Bad

335.83

982.79

2.93

Bax

516.54

1567.29a

3.03

Bim

192.61

1488.21

7.73

Bcl-2

192.61

968.65 a

5.03

Bcl-w

192.61

1342.08

6.97

IGF-1

192.61

984.35

5.11

IGF-2

192.61

997.97

5.18

Caspase 3

192.61

1262.99

6.56

Caspase 8

192.61

1708.19

8.87

HtrA2

192.61

1314.83

6.83

Fas

538.63

1201.19

2.23

FasL

521.06

1154.57

2.22

P53

489.92

1175.53

2.40

P27

620.96

1405.44

2.26

  1. The boldface letters were anti-apoptosis proteins and the others were apoptosis proteins
  2. aThe ratio of Bax/Bcl-2 is 0.60
  3. Up-regulation of fourteen differential proteins expression was more than doubled in the BF + GCV group unlike the expression in the PBS + GCV group. To summarize, the anti-apoptosis proteins were increased more than 5.0-fold. The total ratio of pro-apoptosis to anti-apoptosis proteins was 2.02-fold and the ratio of Bax/Bcl-2 was 0.60. The typical mitochondrial control signal molecule, Cytochrome C (Cyto C), was not significantly changed in group BF + GCV. The results suggested that bifidobacterium itself (not GCV) induced cancer cell apoptosis via Fas/FasL signaling pathway without mitochondrial alteration and up-regulated P53 expression