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Fig. 1 | BMC Cancer

Fig. 1

From: Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors

Fig. 1

Construction and verification of thymidine kinase expression system and GCV phosphorylated by rTK in Bifidobacterium infantis. HPLC detected the concentration of GCV treated with PBS, BF and BF-rTK for 1 h at 37 °C respectively. The supernatants were analyzed by HPLC with octadecylsilane chemically bonded silica column. The mobile phase was methanol + H2O (5 + 95) and UV detection wavelength was 252 nm. a PCR detection of rTK gene in BF-rTK (lane 1 & 2) and BF (lane 3). b PBS + GCV. c BF-rTK + GCV. d BF + GCV. e The BF cell lysis and BF culture supernatant were analyzed by HPLC after incubation with GCV. The BF-rTK supernatant assay suggesting that rTK expressed in BF-rTK cells can be secreted into supernatant (*P < 0.05 statistically significant when comparing treated versus control)

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