Fig. 6

HDAC 1 and HIF positively correlate in clinical ccRCC. a) HDAC 1 expression in TMA was examined by immunohistochemistry. HDAC 1 positive nuclei were quantitated using Image J software and percent positive nuclei (indicated by brown staining) were used for further analysis. Black arrows indicate positive nuclei that were identified by the Image J software. The scale bar indicates 200 μM and the image was captured at 4X using the Aperio software. b) The association between HDAC1 and HIF1 & HIF2 statuses was examined using Wilcoxon rank sum and Kriskall-Wallis exact tests. All analyses were conducted in SAS v9.3 (Cary, NC) at a significance level of 0.05. c) Kaplan Meir curves (overall survival) of unaltered and upregulated HDAC 1 mRNA levels in the TCGA data are shown. The blue line indicates unaltered HDAC 1 mRNA and the red line indicates upregulated HDAC 1 mRNA levels. d) The table shows the numbers of patients in each group and survival data in months