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Fig. 5 | BMC Cancer

Fig. 5

From: Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells

Fig. 5

SSd blocked ATP production, mitochondrial oxygen consumption and extracellular acidification of HSC-T6 cells. (a) HSC-T6 cells were treated with a series of SSd concentrations, and the subsequent cellular ATP production was detected by the Mitochondrial ToxGlo assay. (b and c) The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were detected by a Seahorse XF24 bioenergetic assay. The arrow points denote the injection of SSd, oligomycin (Oligo), FCCP and rotenone/myxothiazol (AA). Steady-state OCR and ECAR were measured before the SSd injection. Oligomycin was injected at the twelfth time point, while maximal oxygen consumption was measured at the fifteenth time point after FCCP injection. (d and e) The data of the 5th time point of OCR and ECAR after SSd injection indicate that SSd (0.5 μM) had stronger inhibitory effects on ECAR than on OCR. The data are the mean ± S.D. from 3 independent experiments. *P < 0.01 versus the control group

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