Skip to main content

Table 4 Operational analysis of two-step KRAS mutation testing of 120 CRC samples

From: A rational two-step approach to KRAS mutation testing in colorectal cancer using high resolution melting analysis and pyrosequencing

  Run 1 Run 2 Summary
HRM n % n % n %
 Number of samples 120 100.0 20 100.0 (16.7) 120 100.0
 Analysis passed 89 74.2 18 90.0 105 87.5
  WT (total) 60 67.4 12 66.7 71 67.6
  WT (skewed HRM curve) 3 5.0 2 16.7 4 5.6
  Mutant (total) 29 32.6 6 33.3 34 32.4
  Mutant (skewed HRM curve) 1 3.4 1 16.7 1 2.9
 Analysis failed 31 25.8 2 10.0   
Pyrosequencing n % n % n %
 Number of samples 19 100.0 (15.8) 3 100.0 (2.5) 19 100.0
 Analysis passed 18 94.7 2 66.7 18 94.7
  WT 12 66.7 0 0.0 13 72.2
  Potential low level mutation 2 11.1 1 50.0 0 0.0
  Mutant 4 22.2 1 50.0 5 27.8
 Analysis failed 1 5.3 1 33.3 1  
Combined HRM + Pyrosequencing      n %
 Number of samples      120 100.0
 Number of HRM runs      140 116.7
 Number of pyrosequencing runs      22 18.3
 Analysis passed      119 99.2
 WT      81 68.1
 Mutant      38 31.9
 Analysis failed      1 0.8