| Run 1 | Run 2 | Summary | |||
---|---|---|---|---|---|---|
HRM | n | % | n | % | n | % |
 Number of samples | 120 | 100.0 | 20 | 100.0 (16.7) | 120 | 100.0 |
 Analysis passed | 89 | 74.2 | 18 | 90.0 | 105 | 87.5 |
  WT (total) | 60 | 67.4 | 12 | 66.7 | 71 | 67.6 |
  WT (skewed HRM curve) | 3 | 5.0 | 2 | 16.7 | 4 | 5.6 |
  Mutant (total) | 29 | 32.6 | 6 | 33.3 | 34 | 32.4 |
  Mutant (skewed HRM curve) | 1 | 3.4 | 1 | 16.7 | 1 | 2.9 |
 Analysis failed | 31 | 25.8 | 2 | 10.0 |  |  |
Pyrosequencing | n | % | n | % | n | % |
 Number of samples | 19 | 100.0 (15.8) | 3 | 100.0 (2.5) | 19 | 100.0 |
 Analysis passed | 18 | 94.7 | 2 | 66.7 | 18 | 94.7 |
  WT | 12 | 66.7 | 0 | 0.0 | 13 | 72.2 |
  Potential low level mutation | 2 | 11.1 | 1 | 50.0 | 0 | 0.0 |
  Mutant | 4 | 22.2 | 1 | 50.0 | 5 | 27.8 |
 Analysis failed | 1 | 5.3 | 1 | 33.3 | 1 |  |
Combined HRM + Pyrosequencing | Â | Â | Â | Â | n | % |
 Number of samples |  |  |  |  | 120 | 100.0 |
 Number of HRM runs |  |  |  |  | 140 | 116.7 |
 Number of pyrosequencing runs |  |  |  |  | 22 | 18.3 |
 Analysis passed |  |  |  |  | 119 | 99.2 |
 WT |  |  |  |  | 81 | 68.1 |
 Mutant |  |  |  |  | 38 | 31.9 |
 Analysis failed |  |  |  |  | 1 | 0.8 |