|  | HRM | Pyrosequencing | |||||
---|---|---|---|---|---|---|---|---|
 |  |  |  | Run 1 | Run 2 |  | ||
Sample | cDNA [ng/μl] | Run 1 | Run 2 | Result | % mut. Alleles | Result | % mut. Alleles | Final result |
62 | 541 | WT a | Â | WT | Â | Â | Â | WT |
63 | 29 | WT a | Â | WT | Â | Â | Â | WT |
64 | 378 | WT | Â | Â | Â | Â | Â | WT |
65 | 342 | WT a | WT | Â | Â | Â | Â | WT |
66 | 176 | failed | WT a | G12V | 3.2 | G12V | 5.2 | WT |
67 | 139 | mut | Â | Â | Â | Â | Â | mut |
68 | 520 | WT | Â | Â | Â | Â | Â | WT |
69 | 42 | WT | Â | Â | Â | Â | Â | WT |
70 | 202 | WT | Â | Â | Â | Â | Â | WT |
71 | 157 | mut | Â | Â | Â | Â | Â | mut |
72 | 259 | mut | Â | Â | Â | Â | Â | mut |
73 | 145 | WT | Â | Â | Â | Â | Â | WT |
74 | 21 | failed | failed | WT | Â | Â | Â | WT |
75 | 22 | failed | Â | WT | Â | Â | Â | WT |
76 | 66 | mut | Â | Â | Â | Â | Â | mut |
77 | 55 | mut | Â | Â | Â | Â | Â | mut |
78 | 199 | mut | Â | Â | Â | Â | Â | mut |
79 | 197 | WT | Â | Â | Â | Â | Â | WT |
80 | 171 | WT | Â | Â | Â | Â | Â | WT |
81 | 55 | failed | Â | G12V | 41.1 | Â | Â | mut |
82 | 231 | failed | Â | WT | Â | Â | Â | WT |
83 | 250 | failed | Â | WT | Â | Â | Â | WT |
84 | 57 | failed | WT a | Â | Â | Â | Â | WT |
85 | 21 | mut | Â | Â | Â | Â | Â | mut |
86 | 248 | WT | Â | Â | Â | Â | Â | WT |
87 | 258 | WT | Â | Â | Â | Â | Â | WT |
88 | 83 | failed | Â | failed | Â | failed | Â | N/A |
89 | 38 | failed | Â | WT | Â | Â | Â | WT |
90 | 279 | WT | Â | Â | Â | Â | Â | WT |
91 | 122 | failed | Â | WT | Â | Â | Â | WT |
92 | 129 | failed | Â | Low Mut. | 5.2 | Low Mut. | 8.2 | mut |
93 | 58 | WT | Â | Â | Â | Â | Â | WT |
94 | 129 | WT | Â | Â | Â | Â | Â | WT |
95 | 254 | WT | Â | Â | Â | Â | Â | WT |
96 | 373 | WT | Â | Â | Â | Â | Â | WT |
97 | 158 | WT | Â | Â | Â | Â | Â | WT |
98 | 96 | mut | Â | Â | Â | Â | Â | mut |
99 | 22 | WT | Â | Â | Â | Â | Â | WT |
100 | 30 | mut | Â | Â | Â | Â | Â | mut |
101 | 26 | muta | muta | G13D | 7.3 | Â | Â | mut |
102 | 49 | WT | Â | Â | Â | Â | Â | WT |
103 | 47 | WT | Â | Â | Â | Â | Â | WT |
104 | 43 | WT | Â | Â | Â | Â | Â | WT |
105 | 54 | WT | Â | Â | Â | Â | Â | WT |
106 | 363 | failed | mut | Â | Â | Â | Â | mut |
107 | 521 | failed | WT | Â | Â | Â | Â | WT |
108 | 199 | mut | Â | Â | Â | Â | Â | mut |
109 | 260 | WT | Â | Â | Â | Â | Â | WT |
110 | 67 | WT | Â | Â | Â | Â | Â | WT |
111 | 103 | WT | Â | Â | Â | Â | Â | WT |
112 | 24 | mut | Â | Â | Â | Â | Â | mut |
113 | 150 | WT | Â | Â | Â | Â | Â | WT |
114 | 5 | WT | Â | Â | Â | Â | Â | WT |
115 | 25 | WT | Â | Â | Â | Â | Â | WT |
116 | 33 | WT | Â | Â | Â | Â | Â | WT |
117 | 26 | mut | Â | Â | Â | Â | Â | mut |
118 | 72 | WT | Â | Â | Â | Â | Â | WT |
119 | 16 | failed | Â | G12V | 12.8 | Â | Â | mut |
120 | 33 | failed | Â | WT | Â | Â | Â | WT |
121 | 48 | WT | Â | Â | Â | Â | Â | WT |
122 | 74 | WT | Â | Â | Â | Â | Â | WT |
123 | 474 | mut | Â | Â | Â | Â | Â | mut |
124 | 431 | mut | Â | Â | Â | Â | Â | mut |
125 | 66 | mut | Â | Â | Â | Â | Â | mut |
126 | 143 | mut | Â | Â | Â | Â | Â | mut |
127 | 49 | failed | WT | Â | Â | Â | Â | WT |
128 | 143 | WT | Â | Â | Â | Â | Â | WT |
129 | 122 | mut | Â | Â | Â | Â | Â | mut |
130 | 139 | WT | Â | Â | Â | Â | Â | WT |
131 | 21 | failed | Â | WT | Â | Â | Â | WT |
132 | 39 | failed | Â | WT | Â | Â | Â | WT |
133 | 128 | failed | Â | G12D | 26.7 | Â | Â | mut |
134 | 60 | mut | Â | Â | Â | Â | Â | mut |
135 | 330 | failed | mut | Â | Â | Â | Â | mut |
136 | 165 | mut | Â | Â | Â | Â | Â | mut |
137 | 213 | mut | Â | Â | Â | Â | Â | mut |
138 | 31 | failed | mut | Â | Â | Â | Â | mut |
139 | 156 | mut | Â | Â | Â | Â | Â | mut |
140 | 59 | WT | Â | Â | Â | Â | Â | WT |
141 | 68 | WT | Â | Â | Â | Â | Â | WT |
142 | 164 | WT | Â | Â | Â | Â | Â | WT |
143 | 238 | mut | Â | Â | Â | Â | Â | mut |
144 | 12 | mut | Â | Â | Â | Â | Â | mut |
145 | 33 | failed | WT | Â | Â | Â | Â | WT |
146 | 81 | WT | Â | Â | Â | Â | Â | WT |
147 | 11 | WT | Â | Â | Â | Â | Â | WT |
148 | 13 | mut | Â | Â | Â | Â | Â | mut |
149 | 71 | WT | Â | Â | Â | Â | Â | WT |
150 | 11 | mut | Â | Â | Â | Â | Â | mut |
151 | 40 | failed | WT | Â | Â | Â | Â | WT |
152 | 50 | WT | Â | Â | Â | Â | Â | WT |
153 | 128 | mut | Â | Â | Â | Â | Â | mut |
154 | 146 | WT | Â | Â | Â | Â | Â | WT |
155 | 69 | WT | Â | Â | Â | Â | Â | WT |
156 | 182 | WT | Â | Â | Â | Â | Â | WT |
157 | 32 | failed | WT | Â | Â | Â | Â | WT |
158 | 142 | WT | Â | Â | Â | Â | Â | WT |
159 | 53 | WT | Â | Â | Â | Â | Â | WT |
160 | 91 | failed | WT | Â | Â | Â | Â | WT |
161 | 334 | WT | Â | Â | Â | Â | Â | WT |
162 | 86 | failed | WT | Â | Â | Â | Â | WT |
163 | 61 | WT | Â | Â | Â | Â | Â | WT |
164 | 64 | WT | Â | Â | Â | Â | Â | WT |
165 | 141 | WT | Â | Â | Â | Â | Â | WT |
166 | 271 | WT | Â | Â | Â | Â | Â | WT |
167 | 40 | WT | Â | Â | Â | Â | Â | WT |
168 | 34 | failed | WT | Â | Â | Â | Â | WT |
169 | 29 | failed | failed | WT | Â | Â | Â | WT |
170 | 354 | WT | Â | Â | Â | Â | Â | WT |
171 | 66 | WT | Â | Â | Â | Â | Â | WT |
172 | 43 | failed | WT | Â | Â | Â | Â | WT |
173 | 114 | WT | Â | Â | Â | Â | Â | WT |
174 | 268 | WT | Â | Â | Â | Â | Â | WT |
175 | 107 | WT | Â | Â | Â | Â | Â | WT |
176 | 170 | mut | Â | Â | Â | Â | Â | mut |
177 | 65 | failed | mut | Â | Â | Â | Â | mut |
178 | 31 | failed | mut | Â | Â | Â | Â | mut |
179 | 61 | WT | Â | Â | Â | Â | Â | WT |
180 | 659 | mut | Â | Â | Â | Â | Â | mut |
181 | 34 | WT | Â | Â | Â | Â | Â | WT |