Fig. 6

PAC inhibits the expression/secretion of IL-6 and MCP-1. SFCM from MDA-MB-231 cells either sham treated (DMSO) or challenged with PAC (10 μM) for 24 h were collected and applied onto Human Cytokine Antibody Array membrane. a Blots, the rectangles indicate the spots corresponding to IL-6 and MCP-1, while the arrows indicate differentially expressed cytokines. b Histogram showing secretion levels of the indicated cytokines upon quantification. Error bars represent means ± S.D, *, P ≤ 0.05. c Cells were exposed to DMSO or PAC, and then cell lysates were prepared and used for immunoblotting analysis using specific antibodies against the indicated proteins