Fig. 5

PAC suppresses EMT in breast cancer cells and tumor xenografts. a Cells were seeded in E-16 plates for 24 h, and then were either sham-treated (DMSO) or exposed to PAC (10 μM) for the indicated periods of time. Cell proliferation was monitored in real time using the RTCA-DP xCELLigence System. b Cells were exposed to DMSO or PAC (10 μM) for 24 h, and then were seeded in the CIM-plate with SFM in the upper wells separated by 8 micron pore size PET membrane with thin layer of matrigel basement membrane matrix (invasion) or without (migration). The lower chambers contained complete media as chemoattractant. Cells were incubated in normal culture conditions for 24 h and the migration/invasion were determined using the real time RTCA-DP xCELLigence System. Error bars represent means ± S.D from 3 different experiments; *, P ≤ 0.001. c Cells were either sham-treated (DMSO) or challenged with PAC (10 μM) for 24 h, and then cell lysates were prepared and used for Immunoblotting analysis using antibodies against the indicated proteins. d Figure legends as in Fig. 4c