Fig. 3

ERα down-regulation potentiates the pro-apoptotic effect of PAC. MCF-7 cells were transfected with vectors containing 3 different ESR1-shRNA sequences (1, 2, 3) or a scrambled sequence (Scbl). a Cell lysates were prepared and used for immunoblotting analysis using antibodies against the indicated proteins. b Cells were treated with PAC (10 μM) and the proportion of apoptotic cells was determined using annexinV/PI-Flow cytometry. c Histogram, Error bars represent means ± S.D from three different experiments, *, P ≤ 0.005