Table 2 Specifications of antigens and corresponding antibodies

PER1 (Per12-A)

Polyclonal

AH Diagnostics AS Fjellgata 1, Oslo

1:50, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

PER2 (N-19, sc-7728)

Polyclonal

Santa Kruz Biotecnology Inc. Europe

1:200, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

PER3 (Per32-A)

Polyclonal

AH Diagnostics AS Fjellgata 1, Oslo

1:50, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

CRY1 (W-L5, sc-101006)

Monoclonal

Santa Kruz Biotecnology Inc. Europe

1:200, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

CRY2 (P-21, sc-130731)

Polyclonal

Santa Kruz Biotecnology Inc. Europe

1:200, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

BMAL 1 (LS-B660/12275)

Polyclonal

Lifespan Biosciences (Nordic biosite)

1:100, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

CLOCK (LS-B278/18928

Polyclonal

Lifespan Biosciences (Nordic biosite)

1:500, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

Anti-CSNK1α1L

Polyclonal

Abcam.com England

1:150, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

Casein kinase 1Ɛ (Sc-25423)

Polyclonal

Santa Kruz Biotecnology Inc. Europe

1:100, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6

Casein kinase 1α (Sc-28886)

Polyclonal

Santa Kruz Biotecnology Inc. Europe

1:100, overnight at 4 °C

Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6