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Table 2 Specifications of antigens and corresponding antibodies

From: Expression of circadian clock genes and proteins in urothelial cancer is related to cancer-associated genes

Antigen Specificities Purchaser Dilution Pre-treatment
PER1 (Per12-A) Polyclonal AH Diagnostics AS Fjellgata 1, Oslo 1:50, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
PER2 (N-19, sc-7728) Polyclonal Santa Kruz Biotecnology Inc. Europe 1:200, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
PER3 (Per32-A) Polyclonal AH Diagnostics AS Fjellgata 1, Oslo 1:50, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
CRY1 (W-L5, sc-101006) Monoclonal Santa Kruz Biotecnology Inc. Europe 1:200, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
CRY2 (P-21, sc-130731) Polyclonal Santa Kruz Biotecnology Inc. Europe 1:200, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
BMAL 1 (LS-B660/12275) Polyclonal Lifespan Biosciences (Nordic biosite) 1:100, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
CLOCK (LS-B278/18928 Polyclonal Lifespan Biosciences (Nordic biosite) 1:500, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
Anti-CSNK1α1L Polyclonal Abcam.com England 1:150, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
Casein kinase 1Ɛ (Sc-25423) Polyclonal Santa Kruz Biotecnology Inc. Europe 1:100, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6
Casein kinase 1α (Sc-28886) Polyclonal Santa Kruz Biotecnology Inc. Europe 1:100, overnight at 4 °C Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6