Fig. 4

Functional consequences of ectopic ROR2 expression in RKO and SW620 cell lines. a ROR2 qRT-PCR of RKO and SW620 cell lines with ectopic ROR2 expression (pROR2 transfection) and control (pFLAG-CMV-4™ transfection) relative to expression in HCT116 cell lines. All expression results normalised against 3 housekeeping genes (n = 1). b CCK-8 proliferation assay of RKO and SW620 cells with and without ectopic ROR2 expression (n = 3). c Wound healing assay comparing percentage area of wound covered by RKO cells with and without ectopic ROR2 expression over a 4 day period (n = 1). d Images of RKO cells in wound healing assay on day 0 and day 3 comparing cells with and without ectopic ROR2 expression. e Wound healing assay comparing percentage area of wound covered by SW620 cells with and without ectopic ROR2 expression over a 12 day period (n = 1). f Images of SW620 cells in wound healing assay on day 0 and day 9 comparing cells with and without ectopic ROR2 expression