Fig. 3

Increased proliferative, metastatic and invasive potential following ROR2 knockdown in HCT116 cells. a qRT-PCR of Wnt & EMT associated genes in HCT116 cell lines after ROR2 siRNA knockdown. All expression results normalised against 3 housekeeping genes (n = 3) (P < 0.05). b CCK-8 proliferation assay of HCT116 cells with and without ROR2 siRNA knockdown (n = 3) (P < 0.01). c Images of transwell migration assay of HCT116 cells with and without ROR2 siRNA knockdown at 10× magnification. d Average cell count comparison between HCT116 cells with and without ROR2 siRNA knockdown. Average count taken from 4 independent image fields at 20× magnification (n = 3). e Images of transwell invasion assay of HCT116 cells with and without ROR2 siRNA knockdown at 10× magnification. f Average cell count comparison between HCT116 cells with and without ROR2 siRNA knockdown. Average count taken from 4 independent image fields at 20× magnification (n = 3) (P < 0.01)