Fig. 2

ROR2 promoter hypermethylation and silencing in adenomas and patient tumour samples. a Matching normal and tumour samples from TCGA database showing differences in ROR2 expression as assessed using Agilent microarray (n = 12) (P < 0.01). b ROR2 methylation comparison in entire cohort of tumour and normal samples from TCGA database as assessed using Illumina Infinium (HumanMethylation450) arrays (n = 258) (P < 0.001). Methylation values were obtained by averaging the beta values of the methylation probes that fell within the ROR2 CpG island. c Average normalised ROR2 expression in entire cohort of tumour and normal samples from TCGA database as assessed using Illumina RNA-Seq (n = 258) (P < 0.05). d Methylation percentages in colorectal adenomas and normal samples as analysed using COBRA assays (n = 47 & n = 88 respectively). e Comparison of ROR2 expression to methylation in colorectal tumour samples from TCGA database (n = 239) (P < 0.0001). Samples with average beta values <0.25 were categorised as low methylation whilst samples with average beta values >0.25 were categorised as high methylation. The results shown here are based upon data generated by the TCGA Research Network: http://cancergenome.nih.gov/. f qRT-PCR of 6 patient adenoma samples with matching normal tissue showing differences in ROR2 expression. Expression was normalised against 3 housekeeping genes. Bisulphite sequencing revealing a corresponding change in ROR2 promoter methylation between samples of patient adenomas and adjacent normal tissue