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Fig. 1 | BMC Cancer

Fig. 1

From: ROR2 is epigenetically inactivated in the early stages of colorectal neoplasia and is associated with proliferation and migration

Fig. 1

ROR2 expression loss in colorectal cancer cell lines caused by promoter hypermethylation. a qRT-PCR of 30 different colorectal cancer cell lines showing ROR2 expression normalised against 3 housekeeping genes. Insert shows the relative position of ROR2 qRT-PCR primers relative to ROR2 gene. b Bisulphite sequencing of 7 colorectal cancer cell lines (C170, HCT116, SW480, SNUC2B, HCT15, SW620, RKO) showing increased methylation index (MI) of ROR2 promoter correlating with decreased levels of ROR2 mRNA expression. Black squares represent methylated CpG dinucleotides. White squares represent unmethylated CpG dinucleotides. Grey squares represent CpG dinucleotide with an inconclusive finding. Gene map of ROR2 indicates the region of the ROR2 CpG island analysed in bisulphite sequencing. c qRT-PCR of RKO and SW620 cells after 5-aza-2-deoxycytidine (5-aza-dC) treatment compared with control cells (n = 3). ROR2 expression was normalised against 3 housekeeping genes. Corresponding bisulphite sequencing reveals loss of ROR2 promoter methylation and decreased methylation index (MI) resulting from 5-aza-dC treatment

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