Fig. 6
From: PIK3CA dependence and sensitivity to therapeutic targeting in urothelial carcinoma
Flow cytometric analysis of apoptotic effects of GDC-0941 in bladder cancer cell lines and TERT-NHUC. a. Examples of FACS Annexin V/PI assay analysis of induction of apoptosis in cells treated with DMSO (nontreated) or 1 μM GDC-0941 for 48 h. X-axis, Annexin V; Y axis, Propidium Iodide (PI). Lower-right quadrant; Annexin V+ and PI- (early apoptotic cell population). b. Quantification of fold difference of early apoptotic cells in population treated with 1 μM GDC-0941 relative to nontreated cells from replicate assays to those shown in A. Values represent mean ± S.E and are representative of quadruplicate experiments. * indicates statistical significant difference (t test, P < 0.05)