Fig. 5
From: PIK3CA dependence and sensitivity to therapeutic targeting in urothelial carcinoma
Flow cytometric analysis of cell cycle effects of GDC-0941 in bladder cancer cell lines and TERT-NHUC. a. Examples of FACS analysis of cells treated with DMSO vehicle (nontreated) or 1 μM GDC-0941 for 48 h. X-axis; DNA content assessed by propidium idodide staining and Y-axis; relative cell number. The legend to the right of each plot shows the percentage of cells in G1, S, and G2/M phase of the cell cycle. b-d. Quantification of replicate cell cycle assays as shown in (a, b). Fold difference of G1. c. Fold difference in S phase. d. Fold difference in G2/M. Values represent mean ± S.E and are representative of quadruplicate experiments. * indicates statistical significant difference (t test, P < 0.05)