Fig. 4

Activity of GDC-0941 in relation to PI3K pathway mutational status in bladder cancer cell lines. A panel of 17 bladder cancer cell lines and TERT-NHUC were treated with a range between 0–2 μM of GDC-0941 for 72 h and viability assessed. a. IC₅₀ values shown as mean ± S.E, representative of triplicate experiments. b. Mutation status of cell lines. Grey represents presence of known mutation. c. Dot plot illustrating GDC-094-sensitive cell lines is linked to mutant PIK3CA status (grey and white circles represent mutant and WT PIK3CA cell lines, respectively). d. Immunoblot showing phospho-AKT (upper band) and total AKT (lower band) in cells treated with DMSO (−) or 1 μM GDC-0941 (+) for 1 h