Fig. 1

p110α and pAKT protein expression VM-CUB-3, BFTC909 and 253J cells with PIK3CA knockdown. a. Immunoblots showing p110α protein expression in cells transduced with shRNAs targeting PIK3CA (KD1 and KD2), non-specific control shRNA (NS) and control empty vector (C), and levels of phospho(p)AKT (Ser473) as read out of PI3K-AKT pathway activation. Cells were maintained in serum-supplemented conditions. b. Quantification of p110α expression relative to tubulin. c. Quantification of pAKT levels relative to total AKT. Results are representative of triplicate experiments. * indicates statistical significant difference from controls (Adjusted, P < 0.05) according to Student’s t test, adjusted using Bonferroni’s test for multiple comparisons. Values represent mean ± S.E