Fig. 2

Knockdown of TUG1 increases cell proliferation in NSCLC cells. NSCLC cells were transfected with shRNA A2 and B2 for the TUG1 knockdown, and scramble RNA was used as the control. The efficiencies of the TUG1 knockdown were assessed through real-time qRT-PCR in three independent experiments. a and (b) shRNA A2 and B2 demonstrated effective knockdown efficacy in H520 and H1299 cells. c and (d) Cell viabilities were examined in shRNA A2 and B2 knockdown H520 and H1299 cells. Significantly increased cell viabilities were noted compared with the scramble RNA in the three independent experiments (*p < 0.05)