Protein | Antigen retrieval | Positive Control | Peroxidase inactivation | Detection system | Antibody | ||
---|---|---|---|---|---|---|---|
Company | Dilution | Incubation period | |||||
MCT1 | Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min. | Colon carcinoma | 0.3 % H2O2 in methanol, 30 min. | R.T.U. VECTASTAIN® Elite® ABC Kit (Vector Laboratories) | Chemicon Ref. AB3538P | 1:300 | Overnight |
MCT4 | Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min. | Colon carcinoma | 3 % H2O2 in methanol, 30 min. | Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) | Santa Cruz Biotechnology Ref. sc-50329 | 1:200 | 2 h |
CD147 | EDTA (1 mM, pH = 8) 98 °C; 15 min. | Colon carcinoma | 3 % H2O2 in methanol, 10 min. | Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) | Zymed Ref. 18-7344 | 1:500 | 2 h |
GLUT1 | Citrate buffer (10 mM, pH = 6.0) 98 °C; 10 min. | Skin | 3 % H2O2 in methanol, 10 min. | Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) | Abcam Ref. ab15309-500 | 1:500 | 2 h |