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Table 1 Detailed aspects of the immunocytochemical and immunohistochemical procedure used to visualize the different proteins

From: Significance of glycolytic metabolism-related protein expression in colorectal cancer, lymph node and hepatic metastasis

Protein Antigen retrieval Positive Control Peroxidase inactivation Detection system Antibody
Company Dilution Incubation period
MCT1 Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min. Colon carcinoma 0.3 % H2O2 in methanol, 30 min. R.T.U. VECTASTAIN® Elite® ABC Kit (Vector Laboratories) Chemicon Ref. AB3538P 1:300 Overnight
MCT4 Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min. Colon carcinoma 3 % H2O2 in methanol, 30 min. Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) Santa Cruz Biotechnology Ref. sc-50329 1:200 2 h
CD147 EDTA (1 mM, pH = 8) 98 °C; 15 min. Colon carcinoma 3 % H2O2 in methanol, 10 min. Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) Zymed Ref. 18-7344 1:500 2 h
GLUT1 Citrate buffer (10 mM, pH = 6.0) 98 °C; 10 min. Skin 3 % H2O2 in methanol, 10 min. Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation) Abcam Ref. ab15309-500 1:500 2 h