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Table 1 Detailed aspects of the immunocytochemical and immunohistochemical procedure used to visualize the different proteins

From: Significance of glycolytic metabolism-related protein expression in colorectal cancer, lymph node and hepatic metastasis

Protein

Antigen retrieval

Positive Control

Peroxidase inactivation

Detection system

Antibody

Company

Dilution

Incubation period

MCT1

Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min.

Colon carcinoma

0.3 % H2O2 in methanol, 30 min.

R.T.U. VECTASTAIN® Elite® ABC Kit (Vector Laboratories)

Chemicon Ref. AB3538P

1:300

Overnight

MCT4

Citrate buffer (10 mM, pH = 6.0) 98 °C; 20 min.

Colon carcinoma

3 % H2O2 in methanol, 30 min.

Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation)

Santa Cruz Biotechnology Ref. sc-50329

1:200

2 h

CD147

EDTA (1 mM, pH = 8) 98 °C; 15 min.

Colon carcinoma

3 % H2O2 in methanol, 10 min.

Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation)

Zymed Ref. 18-7344

1:500

2 h

GLUT1

Citrate buffer (10 mM, pH = 6.0) 98 °C; 10 min.

Skin

3 % H2O2 in methanol, 10 min.

Ultravision Detection System Anti-polyvalent, HRP (Lab Vision Corporation)

Abcam Ref. ab15309-500

1:500

2 h