Fig. 3
V-ATPase is localized at the plasma cell membrane in primary and MCL cell lines. Localization of V-ATPase in (a) Z138 control cells (SCRTR), transiently V-ATPase knocked Z138 cells (V-ATPaseTR), and (b) primary MCL cells. Cells were centrifuged on glass slides and stained for V-ATPase. The nuclei were counterstained by DAPI. MCL primary cells were also stained for CD20 to visualize tumor cells. The MCL control sample was not treated with any primary antibody. All staining’s were analyzed using a Nikon ECLIPSE 80i fluorescence microscope