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Fig. 5 | BMC Cancer

Fig. 5

From: Increased sensitivity of African American triple negative breast cancer cells to nitric oxide-induced mitochondria-mediated apoptosis

Fig. 5

a-b) Cell lines (AA and CA TN cells) were treated with various concentrations (1-300μM) of DETA NONOate for 24h after which they were harvested, lysed and subjected to immunoblot analysis for BAX, Bcl2, Mn-SOD, Cu/Zn SOD, NOX4, cyclin D, and PCNA. β-actin was used as control. Experiments were repeated at least three and a representative data is shown. c-f) Cells treated with cycloheximide (CHX) for various concentrations were subjected to immunoblot analysis for BAX, Bcl2, Mn-SOD, Cu/Zn SOD, NOX4, cyclin D, and PCNA. β-actin was used as control

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