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Fig. 3 | BMC Cancer

Fig. 3

From: Increased sensitivity of African American triple negative breast cancer cells to nitric oxide-induced mitochondria-mediated apoptosis

Fig. 3

Nitric oxide–induced apoptosis in AA breast cancer cells was dependent on increase in ROS. a) Cells seeded in 96 well plates were treated with DETA NONOate (1mM) for different time points (6-24h). 4-hydroxy-alkenal levels was measured using Aldetect Lipid Peroxidation Assay kit as per manufacturer’s instruction. Cells were seeded to confluence and treated with DETA NONOate (1mM) for 24h with or without pretreatment (1h) with N acetyl-cysteine (NAC) at various concentrations (10-30mM). b, d) Cell viability was measured by trypan blue exclusion method using hemocytometer for cell counting. c, e) Immunoblot analysis of total cell lysates was performed for Bax (lower panels). Data are represented in Mean ± SD with significant values presented as *p < 0.01 and **p < 0.001

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