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Fig. 1 | BMC Cancer

Fig. 1

From: Galectin-3 interacts with components of the nuclear ribonucleoprotein complex

Fig. 1

Interaction of galectin-3 with hnRNPA2B1 in nuclear extracts. hnRNPA2B1 was identified as an interaction partner of galectin-3 by affinity chromatography (a) and co-immunoprecipitation (b). a Interaction partners of galectin-3 were eluted with lactose from a galectin-3-coupled sepharose column and analysed by mass spectrometry. Among others, two isoforms of hnRNPA2B1 and the splicing auxiliary factor U2AF65 were identified as lactose-dependent interaction partners of galectin-3. Numbering on the x axis correlates with Additional file 1: Table S1. b Co-immunoprecipitation of hnRNPA2B1 with galectin-3. In “Mock” agarose beads control anti-antibodies were used as negative control. c Immuno blot analysis of the second dimension of a Blue Native PAGE of NE. The percentages of polyacrylamide in the first dimension under native conditions are indicated on the top. Antibodies used for immunoblot detection are depicted on the left

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