Fig. 3

Proliferation and chemosensitivity assay results in SS cell lines with FOXM1 knockdown. a The cell lines were transduced with FOXM1 siRNA or a nontargeting control. The real-time quantitative PCR for FOXM1 showed a reduction in FOXM1 transcript at 24 h after transfection. b Western blotting demonstrated that the cell lines transduced with FOXM1 had significantly reduced levels of FOXM1 protein at 48 h after transfection. c Cell lines with FOXM1 siRNA compared to nontargeting control. Significantly decreased proliferation was recognized in the SYO-1 cells at 96 h after transfection. d SiRNA targeting FoxM1 transfected cells had higher sensitivity for DOX, compared with the control. Data are presented as mean ± SD for three independent experiments. *P < 0.05 by by t-test. N.S., not significant