Skip to main content

Table 1 Efficiency of VHLmut and VHLwt primary culture establishment upon isolation of CA9+ and CA9- cells from early passage cultures or primary single cell suspensions

From: Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine

  Cells sorted at P0 to P2 Cells sorted from primary single cell suspension
Tumor ID Passage CA9+ CA9- CA9+ CA9- Unsorted (DSFM)
118c 0 pure T 50/50a pure T 50/50a no line
137c 0 mixture no line pure T pure N no line
138c 0 mixture no line pure T pure N no line
149c   ND ND pure T pure N pure N
162c 0 pure T 50/50a ND ND 50/50a
171c 2 mixture pure N pure T pure N ND
183 1 pure N pure N ND ND pure N
200   ND ND mixture pure N no line
222c 0 mixture pure N pure T pure N pure N
243c 2 pure T mixture ND ND pure N
267 0 pure N pure N ND ND pure N
271c 0 pure T mixture pure T mixture pure N
291c 0 pure N pure N pure T no line ND
294 0 mixture pure N no line ND pure N
323c 0 pure T pure N pure T mixture pure N
364b   ND ND pure T no line no line
373 0 mixture pure N ND ND pure N
400c 0 pure N pure N pure T pure N pure N
407c 0 pure T pure N ND ND pure N
   6 of 16 ccRCC cultures (37.5 %) 12 of 16 normal cultures (75 %) 11 of 13 ccRCC cultures (84.6 %) 8 of 12 normal cultures (66.7 %) 12 of 17 normal cultures (70.6 %)
  1. T tumor, N normal, ND not done
  2. aPatient had a germline VHL mutation, therefore normal cell cultures are heterozygous
  3. bVHLmut cell line only was generated, n = 1
  4. cVHLmut and VHLwt pairs were generated, n = 13