Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine

Table 1 Efficiency of VHLmut and VHLwt primary culture establishment upon isolation of CA9+ and CA9- cells from early passage cultures or primary single cell suspensions

	Cells sorted at P0 to P2			Cells sorted from primary single cell suspension
Tumor ID	Passage	CA9+	CA9-	CA9+	CA9-	Unsorted (DSFM)
118^c	0	pure T	50/50^a	pure T	50/50^a	no line
137^c	0	mixture	no line	pure T	pure N	no line
138^c	0	mixture	no line	pure T	pure N	no line
149^c		ND	ND	pure T	pure N	pure N
162^c	0	pure T	50/50^a	ND	ND	50/50^a
171^c	2	mixture	pure N	pure T	pure N	ND
183	1	pure N	pure N	ND	ND	pure N
200		ND	ND	mixture	pure N	no line
222^c	0	mixture	pure N	pure T	pure N	pure N
243^c	2	pure T	mixture	ND	ND	pure N
267	0	pure N	pure N	ND	ND	pure N
271^c	0	pure T	mixture	pure T	mixture	pure N
291^c	0	pure N	pure N	pure T	no line	ND
294	0	mixture	pure N	no line	ND	pure N
323^c	0	pure T	pure N	pure T	mixture	pure N
364^b		ND	ND	pure T	no line	no line
373	0	mixture	pure N	ND	ND	pure N
400^c	0	pure N	pure N	pure T	pure N	pure N
407^c	0	pure T	pure N	ND	ND	pure N
		6 of 16 ccRCC cultures (37.5 %)	12 of 16 normal cultures (75 %)	11 of 13 ccRCC cultures (84.6 %)	8 of 12 normal cultures (66.7 %)	12 of 17 normal cultures (70.6 %)

T tumor, N normal, ND not done
^aPatient had a germline VHL mutation, therefore normal cell cultures are heterozygous
^bVHLmut cell line only was generated, n = 1
^cVHLmut and VHLwt pairs were generated, n = 13

ISSN: 1471-2407