Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine

Fig. 1

Generation of patient-matched VHLmut and VHLwt cell cultures from primary ccRCC specimens. a RCC130 primary tissue (left) contained a mixture of wild-type and mutant cells, and growth in 10 % FBS gave rise to a culture of pure VHLmut cells. RCC243 (right) contained a mixture of wild-type and mutant cells, and growth in 10 % FBS gave rise to a culture that still contained a mixture wild-type and mutant cells. b Flow cytometry profiles of CA9 expression in RCC22 VHLwt (left) and RCC22 VHLmut (right). CA9 is expressed on all RCC22 VHLmut cells, but not in RCC22 VHLwt cells. c CA9+ and CA9 cells were sorted from RCC162 cells at passage 1 and placed back into culture, and VHL sequencing was performed after 2 more passages. CA9 cells continued to give rise to a mixed population of mutant and wild-type cells, whereas CA9+ cells gave rise to a culture of pure VHLmut cells. d CD45/CD31-negative cells were sorted from RCC323 primary tumor tissue single cell suspensions into CA9+ and CA9 populations and cultured for 2 passages, then sequenced. CA9 cells gave rise to a culture of pure VHLwt cells, and CA9+ cells gave rise to a culture of pure VHLmut cells

Back to article page