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Fig. 7 | BMC Cancer

Fig. 7

From: Bile acids destabilise HIF-1α and promote anti-tumour phenotypes in cancer cell models

Fig. 7

Dihydroxylated bile acids CDCA and DCA affect the clonogenic ability and cell survival of MCF-7 and DU-145 cells. For clonogenic assays, all cells were grown under hypoxic conditions (DMOG 200 μM), except for untreated cells. A-549 cells (a), MCF-7 cells (b) and DU-145 cells (c) in the presence of 100 μM BAs cells were seeded into 6 well plates and grown over a twenty one day period. Resulting colonies were washed, stained with 0.5 % crystal violet and counted using a steromicroscope. To calculate the surviving fraction (SF), the plating efficiency (PE) was calculated; PE = number of colonies formed/no. of cells seeded × 100 %. This value was used to determine the SF; number of colonies formed after treatment/no. of cells seeded × PE. Clongenic data were derived from three independent experiments, N = 3, S.D; * P < 0.05; ** P < 0.01

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