Fig. 6
From: Bile acids destabilise HIF-1α and promote anti-tumour phenotypes in cancer cell models
Dihydroxylated BAs impair scratch-wound healing in DU-145 cells. For scratch-wound healing assays, all cells were grown under hypoxic conditions (DMOG 200 μM), except for untreated cells. Monolayer cells were grown until 80 % confluent, wounded, stained and images recorded after wounding at 0 and 24 h. Distances between scratch-wound fronts were measured using a steromicroscope and were represented as μm. At least 20 measurement points were taken per condition and averaged data were derived from three independent experiments, N = 3, S.D; * P < 0.05; ** P < 0.01