Fig. 3
From: Bile acids destabilise HIF-1α and promote anti-tumour phenotypes in cancer cell models
Dihydroxylated BAs do not induce apoptosis in DU-145 cells. For apoptosis assays, all cells were grown under hypoxic conditions (DMOG 200 μM), except for untreated cells. DU-145 cells in the presence of 100 μM dihydroxylated BAs (CDCA and DCA) were grown for 16 h and stained with 4′,6-diamidino-2-phenylindole (DAPI) for deoxyribonucleic acid (DNA) (blue) and Annexin V AlexaFluor 488 fluorescence (green). Supplementation of the red pigmented phenoxazinone, cinnabarinic acid (150 μM) to cells acted as positive control. Untreated cells and cells treated with BAs displayed no green fluorescent punctae, whereas positive control cells exhibited green fluorescent punctae indicative of apoptosis at the membrane. Fluorescent microscopy data represent those from one of three independent experiments. Between 10 and 15 images were recorded for each condition. Scale bar = 20 μM. Magnification × 63