Fig. 4

Overexpression of miR-183 inhibits stemness and tumorigenic potential in C666-1 cells. a The expression of stem cell transcription factors NICD3, NICD4, and CYCLIND D1 in the C666-1 cells stably expressing miR-96 and miR-183 was detected by Western blot. Reduced expression of SOX2, OCT4, NICD3, and NICD4 was found in the C666-1 cells with overexpression of miR-96 or miR-183. Similar BMI and CYCLIN D1 expression was observed in the C666-1 cells stably expressing miR-96 and miR-18 and the vector control. Beta-actin was used as the internal loading control. The results were quantified and the histogram shows the fold changes between stable transfected cells and the vector control. b The tumorigenic potential of the C666-1 cells stably expressing miR-96 and miR-183 was assessed by tumor growth in an in vivo nude mice model. Significant inhibition of tumor formation was observed in the C666-1 cells stably expressing miR-183 (**P < 0.01) but not in the cells stably expressing miR-96. Photos reveal the appearances and sizes of the tumors in each treatment group. Student’s t-test was used to determine statistical significance between the two groups (n = 3, **P < 0.01)