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Fig. 8 | BMC Cancer

Fig. 8

From: New anti-cancer chemicals Ertredin and its derivatives, regulate oxidative phosphorylation and glycolysis and suppress sphere formation in vitro and tumor growth in EGFRvIII-transformed cells

Fig. 8

Ertredin suppresses the expression of glycolytic pathway enzymes in NIH/3 T3 EGFRvIII cells under 3D conditions. NIH3T3/EGFRvIII cells (2 × 106 cells/well) were seeded on a ULAS 6-well plate for 3D culture and 10 cm tissue culture-treated plate for 2D cell culture. Ertredin (2 μM), Rotenone (1.25 μM) AG1478 (2 μM) or DMSO (0.1 %) was added to the medium and after 24 h of culture, the cells were harvested and total RNA was isolated. a Microarray heat map. b Relative quantitative charts obtained by qPCR analysis for each glycolysis pathway gene in cells treated with Ertredin (red bar), Rotenone (purple bar), AG1478 (blue bar), and DMSO (black bar) under 3D and 2D conditions. c Lactate in the cells cultured with the indicated substances for 24 h under 3D conditions. 2-deoxyglucose (2DG), a glucose analogue, is known to inhibit hexokinase, the first step of glycolysis. d An explanatory diagram of cell-growth inhibition under 3D conditions by Ertredin

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