Fig. 7

Ertredin inhibited mitochondrial ATP synthesis in NIH3T3/EGFRvIII cells under 3D conditions. a Summary of the assay results. Inhibition of mitochondrial ATP synthesis by 120 min treatment with Ertredin (red line), Rotenone (purple line), AG1478 (blue line) in b NIH3T3/EGFRvIII cells under 3D-culture conditions, c NIH3T3/EGFRvIII cells under 2D-culture conditions, and d NIH3T3 cells under 2D-culture conditions. The cells were cultured in absence of glucose (in galactose-containing medium) and then ATP synthesis was measured. Inhibition of cell growth with the addition of the substances for 3 days in e NIH3T3/EGFRvIII cells under 3D-culture conditions, f NIH3T3/EGFRvIII cells under 2D-culture conditions, and g NIH3T3 cells under 2D-culture conditions